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Pocket Modification of ω-Amine Transaminase Ati>ATA for Overcoming the Trade-Off between Activityand Stability Toward 1-Acetonaphthone Article

Jiaren Cao, Fangfang Fan, Changjiang Lyu, Sheng Hu, Weirui Zhao, Jiaqi Mei, Shuai Qiu, Lehe Mei, Jun Huang

Engineering 2023, Volume 30, Issue 11, Pages 203-214 doi: 10.1016/j.eng.2023.04.009

Abstract: We discovered a structural switch comprising four key amino acid sites (R128, V149, L182, and L187), is 1.7 mmol·L^–1) and half-life (t_1/2) by 59-fold to5.9 L·min^–1·mmol^–1 and by 1.6-fold to 46.9 min, respectively1-acetonaphthone in a 50 mL scaledup system to the desired product, (R)-(+)-1(1-naphthyl)ethylamine((R)-NEA), with 78% yield and high enantiomeric purity (R > 99.5%) within 10 h.

Keywords： Trade-off Co-evolution Amine transaminase (Ri>)-(+)-1(1-naphthyl)ethylamine

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Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1

Frontiers of Environmental Science & Engineering 2023, Volume 18, Issue 4, doi: 10.1007/s11783-024-1808-3

Abstract:

● The first study of electrochemically active magnetotactic bacteria.

Keywords： Magnetotactic bacteria Magnetospirillum magneticumi> Magnetospirillum gryphiswaldensei>

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RR1i> and RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 1, Pages 81-86 doi: 10.15302/J-FASE-2016088

Abstract: As virulence-determining genes, and encode the small subunit and large subunit of viral ribonucleotide reductase (RR) in pseudorabies virus which have been extensively studied in mice. However, their role in pigs has not been adequately investigated. In this study, we deleted and genes based on a / / triple gene-deleted pseudorabies virus and tested its efficacy in pigs as a vaccine candidate. The rescued virus showed similar growth properties and plaque size as its parent strain. In an animal study, the virus could elicit humoral immune responses shown by generation of gB-specific antibodies and virus neutralizing antibodies. However, vaccination could not provide protection against virulent pseudorabies virus challenge since vaccinated pigs showed clinical pseudorabies-specific syndromes. The deficiency in protection may due to the generation of late and low levels of gB antibodies and virus neutralizing antibodies.

Keywords： pseudorabies virus RR1i> and RR2i> ribonucleotide reductase vaccine candidate

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Novel variants in LAMA3 and COL7A1 and recurrent variant in KRT5 underlying epidermolysis bullosa in

Frontiers of Medicine 2022, Volume 16, Issue 5, Pages 808-814 doi: 10.1007/s11684-021-0878-x

Abstract: LAMA3i>, a known recurrent variant (c.74C>T, p.P25L) in KRT5i>, 2 novel (c.2531T>A, p.V844E; c.6811_6814del, p.R2271fs) and 4 known (c.6187C>T, p.R2063W; c.7097G>A, p.G2366D; c.8569G>T, p.E2857*;c.3625_3635del, p.S1209fs) variants in COL7A1 i>were detected.Notably, this study identified a nonsense variant in LAMA3i> that causes EB within the Chinese populationand revealed that this variant resulted in a reduction in LAMA3i> mRNA and protein expression levels

Keywords： epidermolysis bullosa LAMA3i> COL7A1i> KRT5i> Chinese families

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Band-gap tunable (Ga_xi>In₁₋_xi>)₂O₃ layer Research Articles

Fabi Zhang, Jinyu Sun, Haiou Li, Juan Zhou, Rong Wang, Tangyou Sun, Tao Fu, Gongli Xiao, Qi Li, Xingpeng Liu, Xiuyun Zhang, Daoyou Guo, Xianghu Wang, Zujun Qin,zhangfabi@outlook.com,qinzj@guet.edu.cn

Frontiers of Information Technology & Electronic Engineering 2021, Volume 22, Issue 10, Pages 1370-1378 doi: 10.1631/FITEE.2000330

Abstract: Multicomponent oxide (GaIn)O films are prepared on (0001) sapphire substrates to realize a tunable band-gap by technology followed by thermal annealing. The optical properties and band structure evolution over the whole range of compositions in ternary compounds (GaIn)O are investigated in detail. The X-ray diffraction spectra clearly indicate that (GaIn)O films with Ga content varying from 0.11 to 0.55 have both cubic and monoclinic structures, and that for films with Ga content higher than 0.74, only the monoclinic structure appears. The transmittance of all films is greater than 86% in the visible range with sharp absorption edges and clear fringes. In addition, a blue shift of ultraviolet absorption edges from 380 to 250 nm is noted with increasing Ga content, indicating increasing band-gap energy from 3.61 to 4.64 eV. The experimental results lay a foundation for the application of transparent conductive compound (GaIn)O thin films in photoelectric and photovoltaic industry, especially in display, light-emitting diode, and solar cell applications.

Keywords： (GaxIn1−x)2O3薄膜；带隙可调谐；磁控溅射

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Salmonellai>-Delivered COBRA-HA1 Antigen Derived from H1N1 Hemagglutinin Sequences Elicits Broad-Spectrum Article

Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee

Engineering 2024, Volume 32, Issue 1, Pages 42-57 doi: 10.1016/j.eng.2023.08.001

Abstract: antigen (COBRA) was used to generate a consensus sequence of the hemagglutinin globular head portion (HA1)Constructs carrying different HA1 regions were delivered into eukaryotic cells by SalmonellaSpecifically, constructs #1 and #5 increased the production of interferon-γ (IFN-γ) producingCD4⁺ and CD8⁺ T cells, skewing the response toward the T helper type 1 cell (Th1This approach can effectively combat seasonal H1N1 influenza strains and potential pandemic outbreaks

Keywords： COBRA Influenza A Salmonellai> Vaccine Broad spectral protection

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Dihydroartemisinin increased the abundance of by YAP1 depression that sensitizes hepatocellular carcinomato anti-PD-1 immunotherapy

Frontiers of Medicine 2023, Volume 17, Issue 4, Pages 729-746 doi: 10.1007/s11684-022-0978-2

Abstract: Yes-associated protein 1 (YAP1) expression increased in liver tumor cells in early HCC, and AkkermansiaThe response to anti-PD-1 treatment is associated with A. muciniphilai> abundance in many tumorsHere, anti-PD-1 treatment decreased A. muciniphilai> abundance in the colon, but increased YAP1Mechanistically, hepatocyte-specific Yap1i> knockout (Yap1i>^LKO) maintained bileYap1i> knockout enhanced anti-PD-1 efficacy.

Keywords： hepatocellular carcinoma YAP1 Akkermansia muciniphilai> anti-PD-1 dihydroartemisinin bile acid

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NES1i>/KLK10i> and hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Frontiers of Medicine 2019, Volume 13, Issue 6, Pages 646-657 doi: 10.1007/s11684-018-0643-y

Abstract: , we supposed to have an “enhanced firepower” effect by combining overexpressed gene therapy and Itomography/computed tomography (micro-PET/CT) imaging showed that the combined gene therapy and Ithat via inhibition of expression, overexpressed might enhance the effect of radiation therapy of I

Keywords： androgen-independent prostate cancer normal epithelial cell-specific 1/kallikrein 10 sodium/iodide symporter

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Deciphering the Origins of P1-Induced Power Losses in Cu(In_xi>,Ga_1–xi> Article

César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa

Engineering 2020, Volume 6, Issue 12, Pages 1395-1402 doi: 10.1016/j.eng.2019.12.019

Abstract: hyperspectral high-resolution photoluminescence mapping is a powerful tool for the selection and optimiz1ationprocesses used for the patterning interconnections of subcells on Cu(In_x,Ga_{1-Specifically, by analyzing the standard P1 patterning line ablated before the CIGS deposition, we revealWe further rationalize the origins of this effect by comparing the topography of the P1 edge throughWe also investigate the laser-induced damage on P1 patterning lines performed after the deposition of}

Keywords： Cu(Inx Ga1-x)Se2 Cell-to-module efficiency gap P1-induced power losses Hyperspectral photoluminescence

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immune mapped protein 1 (NcIMP1) is a novel vaccine candidate against neosporosis

Xia CUI,Daoyu YANG,Tao LEI,Hui WANG,Pan HAO,Qun LIU

Frontiers of Agricultural Science and Engineering 2015, Volume 2, Issue 1, Pages 66-72 doi: 10.15302/J-FASE-2015047

Abstract: The immune mapped protein 1 (NcIMP1) was identified as a membrane protein, and a previous study indicatedthat NcIMP1 could be a promising vaccine candidate against neosporosis.In this study, the immune response and protection efficacy of NcIMP1 were evaluated.responses against recombinant NcIMP1, with a mixed IgG1/IgG2a response and a predominance of IgG2a productionThe experiment was terminated 30 days p.i., and the cerebral parasite burden in each mouse was assessed

Keywords： Neospora caninumi> immune mapped protein 1 (IMP1) vaccine candidate BALB/c mice

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Successful production of an all-female common carp (Cyprinus carpioi> L.) population using cyp17a1i>-deficient neomale carp Article

Gang Zhai,Tingting Shu,Kuangxin Chen,Qiyong Lou,Jingyi Jia,Jianfei Huang,Chuang Shi,Xia Jin,Jiangyan He,Donghuo Jiang,Xueqiao Qian,Wei Hu,Zhan Yin,

Engineering 2022, Volume 8, Issue 1, Pages 181-189 doi: 10.1016/j.eng.2021.03.026

Abstract: Male and female heterozygous cyp17a1-deficient common carp were first obtained using the clusteredAn all-male phenotype for homozygous cyp17a1-deficient carp, regardless of the individuals&rsquoour laboratory was used to screen the neomale carp population with the XX genotype from the cyp17a1cyp17a1+/+ XX genotype)The average body weight of cyp17a1+/− XX females was higher by 6.60% (8 mpf) and 32.66%

Keywords： Common carp Sexual dimorphism Growth cyp17a1i> Sex steroids All-female population

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Genetic variation of carotenoids in Chinese bread wheat cultivars and the effect of the 1BL.1RS translocation

Wenshuang LI,Shengnan ZHAI,Hui JIN,Weie WEN,Jindong LIU,Xianchun XIA,Zhonghu HE

Frontiers of Agricultural Science and Engineering 2016, Volume 3, Issue 2, Pages 124-130 doi: 10.15302/J-FASE-2016094

Abstract: Total carotenoids, lutein, zeaxanthin and β-carotene were all higher in cultivars with the 1BL.1RS translocation

Keywords： Triticum aestivumi> carotenoids nutritional quality UPLC

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Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressiveFAT1 and inhibits stemness maintenance in thyroid carcinoma

Frontiers of Medicine doi: 10.1007/s11684-023-0999-5

Abstract: Through bioinformatics predictions, we identified that GTF2I and FAT1 were downregulated in thyroid carcinomaThe under-expression of GTF2I and FAT1 was validated in TC cell lines.Ectopically expressed GTF2I and FAT1 were found to augment malignant phenotypes of TC cells, EMT, andMechanistic studies revealed that GTF2I bound to the promoter region of FAT1 and consequently upregulatedMSC-EVs could shuttle GTF2I into TPC-1 cells, where GTF2I inhibited TC malignant phenotypes, EMT, and

Keywords： thyroid carcinoma mesenchymal stem cell extracellular vesicle GTF2I FAT1 CDK4

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Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application

Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li

Frontiers of Chemical Science and Engineering 2019, Volume 13, Issue 3, Pages 554-562 doi: 10.1007/s11705-019-1834-z

Abstract: Using site-directed mutagenesis, we previously obtained a variant of -glucuronidase from Li-3 (PGUS1)In this study, a facile method was established to prepare a CaHPO -PGUS1 hybrid nanoflower for enzymeUnder optimal conditions, 1.2 mg of a CaHPO -PGUS1 hybrid nanoflower precipitate with 71.2% immobilizationand scanning electron microscope results showed that the enzyme was encapsulated in the CaHPO -PGUS1Moreover, the thermostability of the CaHPO -PGUS1 hybrid nanoflower at 55°C was improved, and its half-life

Keywords： βi>-glucuronidase enzyme-inorganic hybrid nanoflower biotransformation glycyrrhizin glycyrrtinicacid 3-Oi>-mono-βi>-D-glucuronide

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APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

Frontiers of Agricultural Science and Engineering Pages 247-261 doi: 10.15302/J-FASE-2021388

Abstract: MdSIZ1 is a SUMO (small ubiquitin-like modifier) E3 ligase that promotes SUMO binding to substrate proteinsTaken together, these results indicate that apple MdSIZ1 negatively regulates drought stress by enhancing

Keywords： apple drought tolerance gene expression MdSIZ1i>

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Title Author Date Type Operation

Pocket Modification of ω-Amine Transaminase Ati>ATA for Overcoming the Trade-Off between Activityand Stability Toward 1-Acetonaphthone

Jiaren Cao, Fangfang Fan, Changjiang Lyu, Sheng Hu, Weirui Zhao, Jiaqi Mei, Shuai Qiu, Lehe Mei, Jun Huang

Journal Article

Electroactivity of the magnetotactic bacteria AMB-1 and MSR-1

Journal Article

RR1i> and RR2i> gene deletion affects the immunogenicity of a live attenuated pseudorabies

Shijun YAN,He YAN,Chaolin ZHANG,Tongyan WANG,Qingyuan YANG,Zhe SUN,Yan XIAO,Feifei TAN,Xiangdong LI,Kegong TIAN

Journal Article

Novel variants in LAMA3 and COL7A1 and recurrent variant in KRT5 underlying epidermolysis bullosa in

Journal Article

Band-gap tunable (Ga_xi>In₁₋_xi>)₂O₃ layer

Fabi Zhang, Jinyu Sun, Haiou Li, Juan Zhou, Rong Wang, Tangyou Sun, Tao Fu, Gongli Xiao, Qi Li, Xingpeng Liu, Xiuyun Zhang, Daoyou Guo, Xianghu Wang, Zujun Qin,zhangfabi@outlook.com,qinzj@guet.edu.cn

Journal Article

Salmonellai>-Delivered COBRA-HA1 Antigen Derived from H1N1 Hemagglutinin Sequences Elicits Broad-Spectrum

Ram Prasad Aganja, Amal Senevirathne, Chandran Sivasankar, John Hwa Lee

Journal Article

Dihydroartemisinin increased the abundance of by YAP1 depression that sensitizes hepatocellular carcinomato anti-PD-1 immunotherapy

Journal Article

NES1i>/KLK10i> and hNISi> gene therapy enhanced iodine-131 internal radiation in PC3 proliferation

Jiajia Hu, Wenbin Shen, Qian Qu, Xiaochun Fei, Ying Miao, Xinyun Huang, Jiajun Liu, Yingli Wu, Biao Li

Journal Article

Deciphering the Origins of P1-Induced Power Losses in Cu(In_xi>,Ga_1–xi>

César Omar Ramírez Quiroz, Laura-Isabelle Dion-Bertrand, Christoph J. Brabec, Joachim Müller, Kay Orgassa

Journal Article

immune mapped protein 1 (NcIMP1) is a novel vaccine candidate against neosporosis

Xia CUI,Daoyu YANG,Tao LEI,Hui WANG,Pan HAO,Qun LIU

Journal Article

Successful production of an all-female common carp (Cyprinus carpioi> L.) population using cyp17a1i>-deficient neomale carp

Gang Zhai,Tingting Shu,Kuangxin Chen,Qiyong Lou,Jingyi Jia,Jianfei Huang,Chuang Shi,Xia Jin,Jiangyan He,Donghuo Jiang,Xueqiao Qian,Wei Hu,Zhan Yin,

Journal Article

Genetic variation of carotenoids in Chinese bread wheat cultivars and the effect of the 1BL.1RS translocation

Wenshuang LI,Shengnan ZHAI,Hui JIN,Weie WEN,Jindong LIU,Xianchun XIA,Zhonghu HE

Journal Article

Extracellular vesicle-carried GTF2I from mesenchymal stem cells promotes the expression of tumor-suppressiveFAT1 and inhibits stemness maintenance in thyroid carcinoma

Journal Article

Construction of a CaHPO4-PGUS1 hybrid nanoflower through protein-inorganic self-assembly, and its application

Tian Jiang, Yuhui Hou, Tengjiang Zhang, Xudong Feng, Chun Li

Journal Article

APPLE SUMO E3 LIGASE MDSIZ1 NEGATIVELY REGULATES DROUGHT TOLERANCE

Baohua CHU, Jia SUN, Huan DANG, Ziqing MA, Shuang ZHAO, Qingmei GUAN, Xuewei LI

Journal Article